Bone and cellular immune system of multiparous sows are insensitive to ovariectomy and nutritive calcium shortage

Autor(en)

Wolfgang Sipos, E Kralicek, M Rauner, C J Duvigneau, H L Worliczek, D Schamall, R T Hartl, I Sommerfeld-Stur, E Dall'Ara, Peter Varga, Herbert Resch, Ilse Schwendenwein, Philippe Zysset, Peter Pietschmann

Abstrakt

Research in osteoporosis, which is a complex systemic disease, demands suitable large animal models. In pigs, most research has been done in growing minipigs, which probably are not ideal models for postmenopausal osteoporosis. Therefore, our aim was to analyze the effects of ovariectomy (OVX) and nutritive calcium shortage on multiparous Large White sows. 32 animals were randomly assigned to 4 groups in a cross design with OVX vs. sham and physiological calcium supplementation (0.75% calcium) vs. dietary calcium shortage (0.3% calcium). The observation period was 10 months with blood sampling every 2 months for hematological, immunological, and biochemical bone marker measurements. At the termination of the experiment, animals were sacrificed. Samples of trabecular bone of distal radius, proximal tibia, and sixth lumbar vertebra were subjected to micro-computed tomography imaging and ashed afterwards. Dual X-ray absorptiometry scans of the proximal femora were performed with prepared bones being placed in a water bath for mimicking soft tissue. Analyses of bone marker and cytokine profile kinetics, distribution of leukocyte subpopulations, and morphometrical and densitometrical analyses showed no evidence of any impact of OVX or calcium shortage. In conclusion, the skeleton of adult sows of a conventional breed is seemingly protected from effects of OVX and calcium shortage.

Organisation(en)

Externe Organisation(en)

Veterinärmedizinische Universität Wien, Technische Universität Dresden, Medizinische Universität Wien, Technische Universität Wien

Journal

Hormone and Metabolic Research

Band

43

Seiten

404-409

Anzahl der Seiten

6

DOI

https://doi.org/10.1055/s-0031-1277154

Publikationsdatum

06-2011

Peer-reviewed

Ja

ÖFOS 2012

403016 Immunologie (Veterinärmedizin), 302019 Geriatrie, 303025 Präventivmedizin, 305902 Gender Medizin

Schlagwörter

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/10fe4024-2374-47e5-b097-8772e6f8f20a