Automated High-Throughput Biological Sex Identification from Archeological Human Dental Enamel Using Targeted Proteomics
- Autor(en)
- Claire Koenig, Patricia Janker-Bortel, Ryan S. Paterson, Barbara Rendl, Palesa P. Madupe, Gaudry B. Troché, Nuno Vibe Hermann, Marina Martínez de Pinillos, María Martinón-Torres, Sandra Mularczyk, Marie Louise Schjellerup Jørkov, Christopher Gerner, Fabian Kanz, Ana Martinez-Val, Enrico Cappellini, Jesper V. Olsen
- Abstrakt
Biological sex is key information for archeological and forensic studies, which can be determined by proteomics. However, the lack of a standardized approach for fast and accurate sex identification currently limits the reach of proteomics applications. Here, we introduce a streamlined mass spectrometry (MS)-based workflow for the determination of biological sex using human dental enamel. Our approach builds on a minimally invasive sampling strategy by acid etching, a rapid online liquid chromatography (LC) gradient coupled to a high-resolution parallel reaction monitoring (PRM) assay allowing for a throughput of 200 samples per day (SPD) with high quantitative performance enabling confident identification of both males and females. Additionally, we developed a streamlined data analysis pipeline and integrated it into a Shiny interface for ease of use. The method was first developed and optimized using modern teeth and then validated in an independent set of deciduous teeth of known sex. Finally, the assay was successfully applied to archeological material, enabling the analysis of over 300 individuals. We demonstrate unprecedented performance and scalability, speeding up MS analysis by 10-fold compared to conventional proteomics-based sex identification methods. This work paves the way for large-scale archeological or forensic studies enabling the investigation of entire populations rather than focusing on individual high-profile specimens. Data are available via ProteomeXchange with the identifier PXD049326.
- Organisation(en)
- Institut für Analytische Chemie, Joint Metabolome Facility
- Externe Organisation(en)
- University of Copenhagen, Centro Nacional de Investigación sobre la Evolución Humana (CENIEH), University College London, Vienna Doctoral School in Chemistry, Medizinische Universität Wien
- Journal
- Journal of Proteome Research
- Band
- 23
- Seiten
- 5107-5121
- Anzahl der Seiten
- 15
- ISSN
- 1535-3893
- DOI
- https://doi.org/10.1021/acs.jproteome.4c00557
- Publikationsdatum
- 2024
- Peer-reviewed
- Ja
- ÖFOS 2012
- 106037 Proteomik, 601003 Archäologie, 104026 Spektroskopie
- Schlagwörter
- ASJC Scopus Sachgebiete
- Allgemeine Chemie, Biochemistry
- Link zum Portal
- https://ucrisportal.univie.ac.at/de/publications/93387de7-46d8-41e2-8d29-bb0c093db050