Methylation analysis of multiple genes in blood DNA of Alzheimer's disease and healthy individuals

Autor(en)

Pierpaola Tannorella, Andrea Stoccoro, Gloria Tognoni, Lucia Petrozzi, Maria Grazia Salluzzo, Alda Ragalmuto, Gabriele Siciliano, Alexander Haslberger, Paolo Bosco, Ubaldo Bonuccelli, Lucia Migliore, Fabio Coppede

Abstrakt

We collected blood DNA from 120 late-onset Alzheimer's disease (AD) patients and 115 healthy matched controls and analysed the methylation levels of genes involved in amyloid-beta peptide production (PSEN1 and BACE1), in DNA methylation (DNMT1, DNMT3A and DNMT3B), and in one-carbon metabolism (MTHFR), searching for correlation with age and gender, with biomarkers of one-carbon metabolism (plasma homocysteine, and serum folate and vitamin B12 levels), and with disease status (being healthy or having AD). We also evaluated the contribution of the APOE ε4 allele, the major late-onset AD genetic risk factor, to the studied gene methylation levels. All the genes showed low mean methylation levels (<5%) in both AD and control DNA, no difference between groups, and no correlation with the studied biomarkers, except for MTHFR that showed methylation levels ranging from 5% to 75%, and correlation with circulating biomarkers of one-carbon metabolism. However, mean MTHFR methylation levels were similar between groups (31.1% in AD and 30.7% in controls, P=0.58). Overall, present data suggest that none of the studied regions is differently methylated in blood DNA between AD and control subjects.

Organisation(en)

Department für Ernährungswissenschaften, Department für Evolutionäre Anthropologie

Externe Organisation(en)

Università degli Studi di Pisa, Associazione Oasi Maria Santissima – ONLUS

Journal

Neuroscience Letters

Band

600

Seiten

143-147

Anzahl der Seiten

5

ISSN

0304-3940

DOI

https://doi.org/10.1016/j.neulet.2015.06.009

Publikationsdatum

07-2015

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Schlagwörter

ASJC Scopus Sachgebiete

Allgemeine Neurowissenschaft

Sustainable Development Goals

SDG 3 – Gesundheit und Wohlergehen

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/05230a10-c90e-48e5-8f87-67cc1d17cbf4