Consumption of anthocyanin-rich beverages affects Nrf2 and Nrf2-dependent gene transcription in peripheral lymphocytes and DNA integrity of healthy volunteers

Autor(en)
Isabel Anna Maria Groh, Tamara Bakuradze, Gudrun Pahlke, Elke Richling, Doris Marko
Abstrakt

Recently, we demonstrated that the consumption of a bolus of bilberry extract modulates the transcription of Nrf2-regulated genes in peripheral blood lymphocytes (PBL) of healthy volunteers, accompanied by decreased DNA-damage. In the present study, we addressed the question whether consumption of consumer-relevant amounts of anthocyanin-rich beverages can achieve similar effects. The impact of three different anthocyanin-rich beverages on Nrf2-dependent gene transcription as well as and the status of DNA-damage in whole blood was investigated. After a polyphenol-reduced diet, five healthy male subjects consumed a bolus (700 mL) of respective test beverages with blood sampling up to 8 h after intake. All beverages affected the transcription of Nrf2, HO-1 and NQO-1, but showed different potencies and persistence of effects. Consumption of red fruit juice significantly reduced total DNA strand breaks (with formamidopyrimidine-DNA-glycosylase-(fpg) treatment) after 8 h in blood samples of the volunteers, suggesting antioxidant and DNA protective effects, albeit transcript levels of Nrf2-dependent genes had reached the basal state. The amount of basic DNA strand breaks (damage without oxidative DNA strand breaks) remained unchanged during the monitoring period. In contrast, a beverage prepared from grape skin extract significantly increased basic and total DNA strand breaks 2 h after intake, underlining the necessity of further investigations regarding composition, safety and consumer ' s acceptance of respective products to exclude undesired adverse effects. Consumption of a bolus of anthocyanin-rich beverages affected Nrf2 and Nrf2-dependent gene transcription in human PBL and DNA integrity, which is indicative for systemic effects.

Organisation(en)
Institut für Lebensmittelchemie und Toxikologie, Department für Mikrobiologie und Ökosystemforschung
Externe Organisation(en)
Technische Universität Kaiserslautern, Eberhard Karls Universität Tübingen
Journal
BMC Chemistry
Band
14
Anzahl der Seiten
10
ISSN
2661-801X
DOI
https://doi.org/10.1186/s13065-020-00690-6
Publikationsdatum
05-2020
Peer-reviewed
Ja
ÖFOS 2012
301211 Toxikologie, 104009 Lebensmittelchemie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/consumption-of-anthocyaninrich-beverages-affects-nrf2-and-nrf2dependent-gene-transcription-in-peripheral-lymphocytes-and-dna-integrity-of-healthy-volunteers(c9f77deb-00a4-4f1c-aca2-0c65a68a131f).html